Research Specialties

Single cell omics - scRNA-seq, scATAC-seq, access of transcription factors (TFs) to DNA-binding motifs within promoters, gene ontology analyses for differentially expressed genes, chromatin access peaks within individual genes, identification of putative ligand-receptor interactions, and cell cycle analyses at single cell resolution.

Systems Neuroscience - combing GFP-labeling of pyramidal neurons via retrograde viruses delivered to the superior colliculus, transgenic RFP-reporter in interneurons, and dual-electrode whole-cell patch clamp recording to map circuits in the cortex.

Fine structure of astrocyte morphology - 3D digital reconstruction from serial blockface scanning electron microscopy.

Mouse genetics - GFP-expressing retinal Muller glia being reprogramming into neurons in vivo by targeted forced expression of the neurogenic bHLH transcription factor Ascl1 and blocking pro-inflammatory signals via inhibition of Sphingosine-1-phosphate receptor 1.

Multiphoton confocal live cell imaging - High-magnification image of axon growth cones

High-resolution imaging of blood vessels in the spinal cord via tissue clearing and light-sheet microscopy

"Brainbow" - Stochastic recombination and expression of different fluorescent proteins to enable structural analyses and identify connections between of individual neurons.